Hi Andrei. I tried, but I couldn't find anything helpful.

Thanks

There are papers using sepharose beads coated with PPD from Mycobacterium bovis for chronic lung infection models in mice, that may give you a place to start. The process we use is coupling the bacterial protein to these sepharose beads. I am not aware of any movies.

Thank you Valerie. Do you have references for those papers?

Thank you

Chensue et al. 2007 should give you a starting place. I am originally from the Chensue lab and have done bead coupling, so if you have specific questions about how to perform the coupling I can answer them. Good luck!

Hi Antonio, contact Dr Alessadra Bragonzi at the San Raffaele Scientific Institute in Milan. Her group makes agar beads routinely and I think she just prepared a video for JOVE

Ciao