Does anybody knowing what sort of techniques/ methods can be used to detect MicroRNA
Hi Mohamed,
There are four main methods, they are microarray, nCounter (NanoString), NGS and qPCR. Microarrays are good for throughput, but lack dynamic range and so accuracy, nCounter is more accurate, but lacks throughput and sensitivity at the lower end. NGS allows discovery of novel miRNAs, but is expensive and require the most data analysis. qPCR is medium price, fast, very accurate but has a medium throughput. The choice of method therefore depends on what you wish to achieve, what sensitivity you need and how much you are wanting to pay for this.
Hi,
this paper may be useful, is old, but give you and idea.
http://www.nature.com/nmeth/journal/v11/n8/full/nmeth.3014.html
As well, be aware that depending on the [] of RNA and sample type you may need to use specific protocols to get a good quality.
hope this help
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