Does anybody have experience with plasmid vectors stably replicating in L. pneumophila or L. micdadei? I have found paper, saying that ColE1 plasmids are able to replicate but what about other E. coli origins like p15a or R6K and bhr derivatives of RSF1010 or pBBR1 (Bordetella bronchiseptica, not Bolivar-Rodriguez)?
Hello- I have not worked with any of those vectors you mention in L. pneumophila- i have worked with pMMB207 derived plasmids, maybe you can determine if those plasmids you want to work with have the same ori? I have never tried moving a plasmid into L. micdadai, so i can't help there.
https://www.addgene.org/vector-database/3600/
in addition, i might just suggest trying them and seeing of they will replicate after electroporation and selection on the correct antibiotic. However, the amount of antibiotics will very for Legionella, so look for papers which use those drugs. I think, for example, AmpR is too common and thus can't be used. We have used Chl and Kan mostly.
thank You for your answer. Vector pMMB207, which you have mentioned is based on RSF1010 origin. I have already tried using different vector based on that ori and indeed it works. Thanks once again!
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