What are some organic solvents that are NOT miscible with acetone?

I am looking an organic solvent that is immiscible with acetone.

03 April 2018 9,487 9 View

What is difference between "statement of teaching philosophy", "teaching statement" and "statement of teaching interests and experience"?

I am curious to know the exact difference between these terms. Thank you in advance for your answers.

08 September 2017 5,794 5 View

What do the Golay plots look like for two GC columns with the same stationary phase but different thicknesses?

I am interested to know about the Golay plots in Gas Chromatography.

03 April 2017 219 3 View

What are the structural and functional differences between microvesicles and exosomes?

04 May 2016 8,408 0 View

What is the mechanistic role of DTT in ligation?

I could not find how DTT helps in ligation. I am looking an exact role of this compound.

11 December 2014 8,649 6 View

Is there any database where I can find all proteins expressed in the liver or any other organs?

I need to find the set of proteins expressed exclusively in liver. Please suggest any database or website.

09 October 2014 6,200 3 View

What is the preferred way of writing "Experimental" or "Experimental Section"?

In scientific writing, some journals write "experimental", while others use "experimental section''. Is there any preference one over the other?

31 December 2013 1,017 4 View

What is 'lumped parameter model' in simple terms?

I am having some difficulty in understanding the concept of lumped parameters analysis. I am not a physicist, would you please explain in simple words.

31 December 2013 4,225 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View