Does anybody know of papers describing markers for mature neutrophils or granulocytes in general?

More Christopher Lee Fogarty's questions See All

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Why do irreversible inhibitors only affect Vmax and not Km?

So I've been wondering why irreversible inhibitors only affect maximal velocity and not Km? Is there a...

19 January 2021 2,509 1 View

In the Venusian atmosphere why doesn't Nitrogen which is much lighter Carbon Dioxide float to the upper regions?

Carbon dioxide on Earth weighs 44 g/mol and has a density of around 1.98 kg/m3, about 1.53 times that of air. Nitrogen gas weighs 28 g/mol and has a density of 1.2506 g/L. During limnic eruptions...

15 January 2021 8,733 4 View

What type of cellular structures are these likely to be?

I have immunohistochemistry slides of human subcutaneous fat biopsy, with skin attached. Most of the structures are self-evident - skin, adipocytes, microvessels in transverse section (revealed by...

03 December 2020 4,238 7 View

Mata-analysis question: How to deal with multiple outcome scales used within individual studies?

Dear all, thanks for the help in advance. I am performing a met-analysis. Most of the studies included report the same outcome using multiple scales e.g. MMSE or CDRS. What is the best way of...

30 October 2020 8,629 5 View

Glucose dehydrogenase interference with glucokinase assay?

Hello all, I'm currently working with undergraduate student-researchers (there are no graduate students at my university) to purify and characterize the drug targetability of glucokinase from pig...

25 October 2020 3,553 7 View

What is the minimum number of timepoints for markov models?

For those who frequently work in markov models, I am interested in knowing the minimum number of time points/observations to get stable estimates of parameters. I have some follow ups, too:...

24 September 2020 9,659 3 View

How best to address statistical differences when there is no difference in the independent variable?

If an experiment includes, say, two groups and Condition 1 is identical between groups but Condition 2 differs between groups, what is the best course of action if one finds a significant...

17 September 2020 9,731 7 View

Can you re-use ethidium bromide post-stain solution more than once, or does ethidium bromide need to be added fresh each time?

When post-staining agarose gels in an ethidium bromide / water bath for 10 minutes, my labmate says she can re-use the post-stain solution 2-3 times before she has to add more ethidium bromide (at...

04 September 2020 680 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Marcin Surmiak

Generally this are neutrophil markers: CD16 CD11b, CD14, CD66b,

http://www.biolegend.com/cell_markers

John Schloendorn

The most frequently used one (in clinical hematology analysis) is... granularity. In other words a particular forward scatter x side-scatter population on a flow cytometer. That works so well, it's sufficient for clinical diagnostic purposes. You can certainly trust the scatter properties alone for research purposes.

Christopher Lee Fogarty

Thanks for the input, but I should have been more clear in my question. I am looking for markers that differentiate immature neutrophils from mature neutrophils. I am familiar with the normal neutrophil markers, but I don't know which are absent on immature neutrophils.

Definitely you should be more specific ;)

As for the neutrophils, "young" neutrophils have low expression of CD16 (but you have to be careful - low CD16 expression can be also observed on apoptotic cells).