Are there any solutions for remote eye-tracking?

Since with the pandemic it doesn't look like we are going back to the lab soon, in my lab we were looking for solutions to conduct remote webcam-based eye-tracking. I've been looking at...

31 January 2021 7,315 5 View

No title

I was working on thermogravimetric analysis for some materials, however, because of the pandmy I stoped using the STA TG DTA-DSC 1600 for like 6 months, when I was able to work again with it, the...

11 October 2020 3,865 2 View

How to get node label from abaqus using python?

I have a model which includes a surface (lets call it EXAMPLE) composed by faces of several 3d elements. Using python scripting, how can I extract (to a text file) the nodes that are part of that...

22 September 2020 9,489 1 View

Which is the best approach for nucleotide substitution model-testing for phylogenetic reconstructions using RADseq data?

I am trying to infer both maximum likelihood and bayesian phylogenetic trees using RADseq reads. I have already created my sets of radtags using Ipyrad which also I used to export the consesus...

24 August 2020 7,823 8 View

Which inputs and outputs should I add on my DEA Analysis?

I'm doing research on the performance efficiency of different oil companies in the world. It has come to my attention that many scholars use DEA Analysis to measure it. However, it is not clear...

23 June 2020 3,223 9 View

Does anyone know of a software to be able to simulate UV lighting / irradiance (300-400 nm) in a given space?

My intention is to use it to calculate the number and distribution of UV lamps within a spherical dome. I need to have a homogeneous distribution and a certain UV irradiance for my experiments....

18 June 2020 1,306 1 View

Retention time variability in HPLC columns specific for PAHs analysis?

We are using a Zorbax HPLC PAHs column and we are observing a high variability in the retention time of PAHs, even in one same batch. Analysis is performed in a LC-MS/MS system, ionization source...

16 April 2020 3,444 8 View

Which environmental problems arise from landfill mining ?

Landfill mining techniques arise as one of the available alternatives to mitigate negative environmental impacts from municipal solid waste landfills. High recycling potential generation is one of...

28 March 2020 1,782 10 View

NetCDF in R and Arcmap?

Hi, I have a problem exporting Netcdf from R 3.6.3 to ArcGIS 10.6. ArcGIS does not recognize the file and eventually closes. The problem is solved only using a previous version of R 3.4.4. Does...

22 March 2020 8,086 3 View

How can I make input file for rehh package in R with scaffolds?

I am trying to look for positive selection in the genome of a non-model specie, therefore I do not have chromosomes structure, I just have scaffolds and positions of SNPs in a VCF file. I used...

11 March 2020 3,700 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View