Hi Refik,

I am not sure if you can use Axograph to do singles analysis but you can definitively do it using Clampex.

Ezequiel

Thanks Ezequiel,

You probably right. I have not done it before, so I though I ask, just in case there is a way around it. Thanks for confirming my thoughts.

cheers, Refik

The parameters you need to calculate the conductance should be straightforward to obtain using axograph? 

Which parameters specifically are you looking to measure?

Simon 

Thanks Simon,

I want to analyze single channel conductane of single hemichannels. There is no conductanca analysis function in Axograph. Have you done single channels conductance analysis before? If yes, perhaps we can meet for a coffee and you can tell me about it.

cheers,

Refik

Hi Refik

I have not specifically done single channel conductance, but I do use axograph regularly so I might be able to help. 

In which lab do you work at QBI?

Simon 

Hi Simon,

I am at the School of Biomedical Sciences, MacGregor Building. I am actually planning to transfer my data to Clampfit which seem to have conductance analysis within the software. If you are around I'd like to meet, since there are not that many electrophsyiologists around these days. Are you from NZ?

Thanks,

refik

Yes I"m from NZ, but working at QBI now.

Sure I'd love to meet a fellow electrophysiologist!

I'm around on Friday afternoon if that suits you?

SImon