Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How to obtain the saturation point of a soil layer from the volumetric soil water content data?

Hi Would you please let me know if the following is accurate as an answer to the question: Suppose we have sensors which measure the volumetric soil water content of a soil layer for a long...

01 August 2020 8,337 3 View

Do have recomendations for open source tools/software in the field hydrology/hydrogeology?

I am doing a small research to review the water related open source tools / software (e.g FREEWAT). if you have used/developed/ heard of similar tools,please share it with me ? Thanks in advance

30 July 2020 4,779 17 View

Can a deep-seated non-piercing diapir impact on aquifer flow system of upper formations?

Can a deep-seated non-piercing diapir impact on aquifer flow system of upper formations? The mentioned diapir affect the upper formations by uplifting them toward the surface and forming some...

22 April 2020 3,031 6 View

How accurate are the SONAR based methods to determine depth of water level in a borewell?

Physically measuring depth of water in a borewell is very difficult. First we need to dissemble the cap of the borewell. Then we need to allow measuring tape inside borewell which is very...

10 March 2020 1,780 3 View

Which software is best for making Gibb's plots?

Please, can you suggest a high graphical resolution software for creating hydrogeochemical graphs, especially for Gibb's diagrams?

20 January 2020 2,623 2 View

Time that rainwater resides on a soil surface?

Is there an average time (or order of magnitude) that rainwater resides on a soil surface? A kind of residence time or ponding time? I am looking at a natural soil (not urban), normal...

16 December 2019 4,365 3 View

Can you suggest any methodology for interpreting the activity radium in groundwater ?

im looking for an hydrogeological methods of interpreting the activity istopes of radium in groundwater and some relation of this isotops with the other physical and chemical parameters,...

09 October 2019 5,932 3 View

Vulnerability intrinsic to groundwater contamination assessed via Electro-Resistivity Method - Vertical Electrical Survey (SEV) for geoelectric layer?

What reference studies (Book, Article, Tesis etc) you know used ER Method was applied using the Vertical Electrical Survey (SEV) technique (Wenner-Schlumberger, dipole-dipole arrays) for...

07 August 2019 5,108 9 View

What is the sinkhole mechanism under a leaked sewer pipeline in a non-krast area (areas with no carbonate rocks or lime stone)?

What is the sinkhole/subsidence/ ground collapse mechanism under a leaking sewer pipeline in a non-krast urban area (areas with no carbonate rocks or lime stone)? Because sinkhole also use to...

07 August 2019 7,279 6 View

How can we Calibrate of Using "Groundwater potential zones method" by wells data? and what type of wells data is needed?

I am working on assessment of groundwater potential zones for my M.Sc thesis I used multi thematic layer for study area and determine their weights using both AHP and MIF methods I need to know...

06 July 2019 9,720 1 View