Please share your experience with LMP IHC.
If we review the literature, most of articles are based on crystalline materials. However we find less amount of literature on applications of amorphous metal oxide materials. Definitely, in solar...
14 July 2021 2,357 4 View
Hello to whomever is reading this. I am working on HPLC analysis of anthocyanins in sweet potato leaves and tubers. One of the methods I have found requires a mobile phase solvent of "5% formic...
03 March 2021 5,244 5 View
as they should not loss nutritional quality
14 June 2019 6,986 1 View
I am looking for the localization of 2 proteins- BRG1 and p53BP1 in HeLa cells. It is supposed to come in nucleus but I am always getting it in cytoplasm. what could be the reason? P.S. I have...
10 September 2016 4,643 3 View
Bemisia tabaci is becoming a serious threat again in cotton. The resurgence issue if any related to present day insecticides viewed to be seriously.Post your experience.
14 July 2016 6,884 6 View
I am building a framework in a .NET C# . where we can compile CUDA program on local machine and execute it on a remote machine, where capable GPU exists. So is there a specific way to achieve...
09 February 2016 3,300 6 View
There are reports of PBW survival in bollgard II Bt cotton hybrids in India. Will it be a potential problems in years to come? How to over come.
28 December 2015 9,355 6 View
How does the contact area of a P-N junction diode (n-ZnO/p-Si) affect the I-V characteristics and rectification ratio? Please suggest some paper and literature also where this kind of study or...
18 July 2015 468 3 View
I want to do selective hydroxylation of methyl group
24 March 2015 9,812 2 View
I would like to prepare protoporphyrin IX dimethyl ester. I would be grateful if anyone could help me out.
04 March 2015 7,719 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
