02 July 2019 4 825 Report

I'm looking for any research done on psychedelic assisted group psychotherapy. The research papers out there seem to be a challenge to access.

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Find a full text article?

Hello. I can´'t find a full text article that i need for my research. Can you help me? The reference of the article is: Ref ID: 3441009. Article: Adolescent Health: Communication With Adolescent...

16 May 2024 3,293 3 View

How to properly extract proteins from a Caco-2 cell line?

Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...

12 May 2024 6,819 2 View

How long do human brain tissue samples last after protein isolation?

Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...

02 May 2024 9,588 3 View

How to get successful bacterial plasmid growth?

I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...

08 April 2024 2,035 4 View

Correlation confusion, am I interpreting this data correctly?

Hello! I have carried out my undergraduate research project using two pre-validated, Likert scale self report questionnaires looking at emotional intelligence and resilience. (SSEIT and...

16 March 2024 7,642 3 View

Undergraduate Statistical research help?

Hi, I am carrying out a very basic undergraduate research study with no stats teaching on the course (medical degree) as a result I am floundering and wonder if anyone could help. I am using two...

01 March 2024 6,741 9 View

How can I have a better transfection rate of 50% for my HT-22 hippocampal neuronal cells?

I am currently working with HT-22 cells by transfecting them with bacterial plasmids to perform IHC. However, we have not had much success with reaching a 50% transfection rate. I usually do about...

17 January 2024 7,802 6 View

Is this normal on my Caco-2 culture?

hi again! I had never worked with Caco-2 cells before and just recently I thaw some. When I look at them I see something that I really don't know what it is. I know these cells secrete a lot of...

14 January 2024 9,068 4 View

How can I fix my transfected cells for immunofluorescence protocol?

Hello. I plan to do a simple immunofluorescence procedure with HT-22 cells. I plan to transfect the cells and fix them. I do not believe I will have time to come in on the weekend. Is it possible...

11 December 2023 6,640 2 View

Powerbead Pro Plates (Qiagen) - leaking?

Hello! Does anyone have experience in solving the issue of leaking due to the film not fully adhering, after following all the instructions from...

19 October 2023 618 0 View

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Dear colleagues, Can anybody by chance have pdfs of two books in Chinese: Liu (2003) and Zhang et al. (2004)?

Liu Zhaosheng. 2003. Triassic and Jurassic sporopollen assemblage from the Kuqa Depression，Tarim Basin of Xinjiang，NW China. Palaeontologia Sinica， New Series A，no. 14（Whole no. 190）....

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Does anyone know the scoring and interpretation for Psychological Birth Order Inventory (PBOI) of White Campbell?

Hi, I am currently a upcoming 4th year student who is need of your help as I couldn't find any accessible file for the manual scoring and interpretation for the PBOI - White Campbell. My group and...

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I ask Chinese seismologists to inform me: who should I contact to create a joint Forecast Center?

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Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

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Is there a validated questionnaire for target audience research?

Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...

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Do you think this contaminations?

Kg1a cell line in 96 well plates do you think this white dots are contaminations or depris ( insides black circle)

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How to set the background of plot to white color in Silvaco Tonyplot?

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I noticed some white growth in the phosphate buffer that was prepared just a week ago. What could be the cause of this, and how can it be resolved?

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