I have some E. coli isolates who are positive to stx1 and stx2 genes by PCR. Now I am interested to know whether my isolates are producing stx1 and stx2 toxins and quantify them. In my lab we have established beads ELISA for cholera toxin but not for stx toxins. So, I am badly in need of anti-stx1 and stx2 antibodies. Do anybody have the antibodies? or how can I get them?
Hi Jayedul Hassan, I have used anti-stx1&2 antibody from KPL its an USA company. Here I'm sending you the link check it out. Good luck!!!
http://www.kpl.com/catalog/productdetail.cfm?Catalog_ID=17&Category_ID=415&Product_ID=1303
Hi Jayedul,
Here you will find both, purified Stx1 and 2 and respective specific antibodies.
https://www.listlabs.com/product-information/shiga-toxins/
Good luck
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