... and his early theories as sort of first indication of CR and sustainable behaviour, especially on non-renewable material?
Natural capital as a theory is widely used. Is there a practical way to use this approach on companies to measure their level of sustainability?
01 February 2015 3,784 4 View
To sort the scanned document image I have to determine the class of document. But no of classes of image will keep on adding even after training. So at time of inference first I will detect unseen...
16 February 2021 4,501 3 View
I have tried to sort CD8+ CXCR5+ in the ARIA but the purity of my sample is about 50%. After sorting, I stain my samples to verify purity but it is low. So, I thought about "downregulation" or...
15 February 2021 8,034 7 View
i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions
15 February 2021 8,939 3 View
Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...
07 February 2021 7,101 1 View
Design for Sustainability (DfS) is a wicked and complex problem. It needs the designer to combine specialized knowledge from several disciplines with systemic thinking. The designer also needs to...
05 February 2021 2,998 9 View
I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...
04 February 2021 6,650 2 View
Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...
04 February 2021 5,173 6 View
Hi, I am currently working on research on communication behavior in Mixed Reality. I am planning a study, where dyads perform a task, in which they have to collaboratively cluster or sort cards...
31 January 2021 4,979 3 View
I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...
21 January 2021 9,722 3 View
I want to learn about mutation in transit peptide. Can mutation in transit peptide affect the function of the protein?
20 January 2021 396 3 View