... and his early theories as sort of first indication of CR and sustainable behaviour, especially on non-renewable material?
Natural capital as a theory is widely used. Is there a practical way to use this approach on companies to measure their level of sustainability?
01 February 2015 3,972 4 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
What are the modern approaches to the organization and sustainable management of greenery in urbanized cities? How can ecological and recreational functions be introduced into the landscape and...
23 July 2024 7,292 4 View
What sustainable management strategies can be used to prevent soil erosion and role of biodiversity in sustainable agriculture?
22 July 2024 5,672 4 View
How can sustainable agriculture practices mitigate climate change and ensure food security and role of agriculture in environmental sustainability?
17 July 2024 1,712 12 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View
It seems that since 2012 Rio +20 Conference, the world has been moving away from science based perfect market thinking, a moved that apparently has been helped by the confusion created by the 2012...
02 July 2024 4,530 4 View
How can technology contribute to environmental sustainability and technology be leveraged to develop innovative solutions for climate change?
26 June 2024 8,501 3 View
I am purifying RNAse E in E. coli (118 kDa). I cloned the rne coding sequence on pET28a with a His tag at the N-terminus. The protein overexpressed fine (see attached figures). The binding buffer...
24 June 2024 6,287 4 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View