Does anybody have a lipase assay protocol for germinating seeds?

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Sumit Kumar

You will have to prepare aqueous extract of seed in suitable buffer and proceed with further lipase assay using method of Kilcawley et al. 2002, using para-nitrophenyl palmitate as substrate. Paper is attached for your kind reference.

Viraj Yele

You can refer attached paper for simple assay of lipase.

Elsayed MOHAMED Elazazi

file attached