We find that each pipette drop of brine shrimp in water deposits a highly variable amount. Does anyone use a tool or technique in their work that could potentially be co-opted to dispense a small predetermined number or weight of tiny brine shrimp?
Hi, if you place the nauplii in a bucket with 10 liters (for example) of aerated saltwater (do not use air stone) then your counts may homogenize. (Take two aliquots of 0.5 ml each and then make a count, there should be no major difference to 10 nauplii between). The central idea is to keep the nauplii in a homogeneous suspension throughout the water column.
Much depends on what is the scale of you experiment? Do you need to standardize number of prey in 1 mL or in 10 L?
Did you use a hemocytometer or a Palmer cell to count the number of individuals/volume? Just homogenize the mixture and add the appropriate volume to bring the number to the desired amount.
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