Dear Snur,

1-You may use the following procedure suggested by Hiba Ali Hasan et al. published in  Pharmaceut Anal Acta 2012, 3:9 http://dx.doi.org/10.4172/2153-2435.1000184:

Soxhlet extraction (SE): The extraction of soluble compounds from Zingiber officinale by the soxhlet method was performed by using methanol or n-hexane as a solvent. The soxhlet procedure consisted of ground ginger rhizomes (30 g) placed inside a thimble loaded into the soxhlet extractor (Gerhardt/Germany). The total extracting time was (10 hrs.), and the total amount of solvent was (300 ml) maintained continuously refluxing over the sample. The solvent assays were performed at solvent boiling temperature. After the extraction the solvent was removed from the solute mixture by reduced pressure with rotary evaporator (Heidolph/Germany).

To view the full publication, please see attached file.

2-Similar solvent (methanol) was used by Xi Shao et al. to extract [10]-shogaol and etc:

J Agric Food Chem. Author manuscript; available in PMC 2012 Sep 19.

Published in final edited form as:

J Agric Food Chem. 2010 Dec 22; 58(24): 12608–12614.

Published online 2010 Nov 23. doi: 10.1021/jf1029256

Quantitative analysis of ginger components in commercial products using liquid chromatography with electrochemical array detection

Abstract

For the first time, a sensitive reversed-phase HPLC electrochemical array method has been developed for the quantitative analysis of eight major ginger components ([6]-, [8]-, and [10]-gingerol, [6]-, [8]-, and [10]-shogaol, [6]-paradol, and [1]-dehydrogingerdione) in eleven ginger-containing commercial products. This method was valid with unrivaled sensitivity as low as 7.3 – 20.2 pg of limit of detection and a range of 14.5 to 40.4 pg of limit of quantification. Using this method, we quantified the levels of eight ginger components in eleven different commercial products. Our results found that both levels and ratios among the eight compounds vary greatly in commercial products.

To view the full publication, please see attached file.

3-Another publication which may be helpful is the one by Suzanna M. Zick, et al.

Int J Biomed Sci. 2010 Sep; 6(3): 233–240.

Quantitation of 6-, 8- and 10-Gingerols and 6-Shogaol in Human Plasma by High-Performance Liquid Chromatography with Electrochemical Detection

Abstract

Zingiber officinale is one of the most commonly used spices. We developed a method to determine the main pungent ginger constituents, 6-, 8- and 10-gingerols and 6-shogaol in human plasma. Quantitation was achieved using a reversed-phase C18 column using high-performance liquid chromatography with electrochemical detection. The assay was linear from 0.1 to 5.0 μg/mL. The within-day coefficients of variation for the assay at 5.0 μg/mL were ≤5% for all analytes. The recovery of all four analytes was ≥99% for at 5.0 μg/mL. The lower limit of quantitation was 0.1 μg/mL except for 10-gingerol which was 0.25 μg/mL. Currently, there is no analytical method for detecting pungent ginger constituents in human plasma. This HPLC method allows for the detection of all four of ginger’s pungent constituents simultaneously in a relatively short run time of 25 minutes. This method should be useful for determining plasma levels of 6-, 8-, 10-gingerol and 6-shogaol in phase I clinical trials.

To view the full paper, please see attached file.

Hoping this will be helpful,

Rafik

Dear Dr Snur, greeting

You can search in my and Hehsu's PhD thesis, the protocols are listed over there.

Best Regards

Thank you, dear Dr. Hemin, already I have it.

   Regards