During pyrolysis I am getting a peak of acetic acid.Is it possible that it is fromacetylene glycol?
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
When planning a randomized, double-blind trial with multiple arms, there are often challenges for blinding the study treatment/groups due to different formulations/packaging/dosage. E.g. Study...
17 December 2023 8,345 1 View
Hello, Greetings of the day, I want to calculate the relative quantum yield w.r.t. quinine sulfate (QS). Before going to sample, I want to ensure my standard (QS) and tried to calculate the...
06 October 2023 6,586 2 View
I am working on peptide molecule for SC delivery for my thesis work. Which solvents (or co-solvent) shall I use other than PEG, PG, Glycerin to increase the solubility (drug loading) of peptide...
15 August 2023 4,834 4 View
I am trying to fluorescently label RNA and purifying the labeled RNA further. The protocol recommends using gel filtration based membrane in the spin columns.
10 August 2023 2,332 1 View
Hello, I am building a prototype transcutaneous auricular vagus nerve stimulator as a part of my PhD. So far, I have only found t-vns.com that have a device with the electrode that targets cymba...
18 May 2023 2,658 0 View
I am generating mRNAs for intra-tumor injections in mice. I want to reduce non-specific immunogenicity due to dsRNA contaminants after IVT. Any simple protocols or kits which could ensure...
04 July 2022 5,817 1 View
Hi everyone, I am performing McMurry coupling reaction of my carbonyl compound. After reaction completion and purification, I found that instead of McMurry dimer, the reduction of ketone happened...
27 March 2022 9,154 3 View
If the Ammonium Hydroxide (NH4OH) is boiled, Will only Ammonia from the soultion would start to evapotrate and Ammonia vapour would be generated at 40°C or the Ammonium Hydroxide vapour?
18 July 2021 3,417 2 View
I am interested in learning about and reviewing new research in the field of data analytics. Where can I find new papers that I can peer review?
19 April 2021 5,443 8 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View