Although   I could not locate any complex between  MB-Creatinine in literature, yet I  could find an  article which gives an idea that there is some sort of reaction between the two which has resulted a decrease in  creatinine content in rats when injected with MB  [ Zoom 200%]..

Thank you so much sir. Do you know of any complex formation between picric acid and methylene blue?

Despite repeated searches on google, I could not find in literature the existence of  any complex between picric acid and  MB.

Thank you so much sir

Greetings Pallavi,

I consider that supramolecular systems (instead of "complexes") are able to assemble through ion/dipolar/hydrogen bond interactions in a subtle balance, therefore you can construct such systems in various stoichiometries with accompanying variation of physicochemical properties/characteristics. Therefore if the supramolecular pairs that you are wondering are not yet published in the literature, then study their interactions and try to mix them in various ways in order to obtain suitable monocrystals for X-ray diffraction/X-ray powder diffraction analysis as well as by FTIR and calorimetry and stare at the obtained results and ways to interact among the two pair in 3D. For further details about my suggestions see our recent contributions in the field of supramolecular pairs:

doi:10.1016/j.colsurfa.2014.04.051

doi:10.1016/j.fuel.2014.08.042

Best regards and happy co-crystallizations.

Hiram I. Beltrán

I think the best way to follow up a MB-creatinine ligand formation is through NMR, weather it might be through hydrogen bonding or mediated by the chromium ion or any other mechanism..

If I were you, I would do a MB-creatinine “titration”,using 1H  NMR, carefully observing the chemical shifts changes of the creatinine protons bonded to both Nitrogens. In this way you would be able to evaluate the  “complex” formation and its stoichiometry as well, that is one of your concerns.

Thank you so much.