we tried to get the surface profile of the untreated sand sample and bacteria treated sand with biopolymers on the surface of the sample in wet condition. we used 3D OSP for this purpose. we got the surface profile of untreated sand sample but for biopolymer coated sand in wet condition does not give any results. The instrument does not sense the sample. we tried 5 times but no result. what will be the problem? how to get surface profile/ surface rough ness of the wet biopolymer coated sand surface?
Note: 3D OSP instrument is working fine.confirmed.