What is the accepted recovery rate range for an acid digestion(HCl:HNO3), followed by an AES analysis?

I'm doing a heavy metal analysis by digesting bird feathers using acids. And in order to verify whether the method i use and the instrument i use give accurate results i was planning to do a...

22 September 2020 4,129 3 View

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?

If I dissolve 35 g of NaCl in 100 ml, what will be the approximate specific gravity?. Is it 1.35? or is there some trick? I read that saturated NaCl soltion has specific gravity of 1.2. Also...

13 May 2020 5,065 2 View

How to avoid uneven (intensity) bands during Western Blotting?

I've uneven bands (size, intensity, and staining) when doing Western Blotting and I'm wondering what the problem could be. The protocol: -10% separating gel (0.375M Tris pH8.8; 0.1% SDS; 10%...

14 July 2019 6,577 8 View

How to ask a good question in RG? and get many responses?

Do we need to be specific? Sometime I feel that if we become specific with more details when we ask the questions, we loose some potential researchers from other fields. So the number of answers...

27 May 2019 3,627 24 View

Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

I would like to know the, Thai Electricity Production percentages (Breakdown according to source, for example Hydropower%, Thermal power%, Solar% etc.)?

27 February 2019 9,882 6 View

Hello.. Is it possible to analyse the cross sectional area propagation of a flame by using mesh free method ?

18 January 2017 9,363 1 View

How to dissolve PEI (polyethylenimine)?

20 April 2016 2,880 22 View

Is there any possibility that tofu protein recovery can achieve more than 100%?

I make tofu from vegetable soybean (edamame). Water to bean ratio for tofu making is 1:12 and CaSO4.2H2O as coagulants. Vegetable soybean has moisture content 64% (wet basis) and protein 5% (wet...

16 October 2015 3,816 12 View

Could someone help me with some Neuronal Tissue Co-IP issues?

I have been doing co-immunoprecipitations for a while now using my own buffers (CHAPS lysis buffer + protease & phosphatase inhibitors) and Dynabeads protein G beads. Useful information: -The...

21 January 2014 5,209 3 View

Evaluation of extraction socket healing

How to evaluate extraction socket healing (when the wound is primarily sutured)? Most of the studies use Landry and Turnbull Healing Index. There is also the H2O2 bubbling test. Do you have any...

01 January 1970 2,794 2 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

How to purify cycle sequencing PCR reaction?

i am purifying cycle sequencing PCR reaction. i have tried multiple methods given in BDT manual (provided with the instrument) .everytime i used 96 well reaction plate for this purpose. 7 wells...

14 February 2021 8,534 2 View

How can I multiply values with standard distribution and with triangular distribution?

I am calculating emissions considering the uncertainty of the data to do Monte Carlo simulation. Some variables such as yields have normal, lognormal or even uniform distributions. These must be...

03 February 2021 6,318 5 View