Do you think SARS-CoV-2 can be establishing a persistent infection like other RNA viruses that can lead to future complications differents from the ones we already know?
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I have performed the Molecular Docking using ADFR suite, I have obtained the files in _out.pdbqt format for ligand docked poses, upon inspection, both visually and reading the pdbqt files of...
08 June 2024 8,130 2 View
I am currently studying index modulation aided NOMA technique but my mind blown up with mathematical expressions. Can someone explain to me these; How Index Modulation aided NOMA (IM-NOMA) works?...
27 April 2024 2,736 1 View
i am working with membrane protein and there is a his tag 4 amino acid above the c terminus (seq HHHHHHDLEY). anti his antibody does not recognise the his tag in western blotting. i was wondering...
14 April 2024 2,675 5 View
I have done the LC-MS result of my complex and its molecular weight is supposed to be 515.5 g/mole. I want someone who is experienced in LC-MS data interpretation. I have attached the picture of...
16 February 2024 382 2 View
quick summary: I am looking for a way to slow down lift-off (1165 or acetone) for a given material system (changing materials to solve the problem is not possible) This is a relatively general...
08 January 2024 8,955 2 View
Dear ResearchGate Support Team, I hope this message finds you well. I am writing to bring to your attention a concerning matter regarding the authorship of certain articles on ResearchGate. It has...
25 November 2023 3,908 1 View
how can we calculate average heterozygocities and unique alleles also how we can compare both populations of different ponds?
21 November 2023 3,238 1 View
i need assistance with few terms your help will be appreciated.
21 November 2023 4,398 1 View
Using machine learning algorithms
26 July 2023 3,025 2 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey
05 August 2024 5,332 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View