Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Why am I getting such large heats of dilution in my Isothermal Titration Calorimetry Experiment?

I am trying to measure binding of calcium to an expressed protein we are studying and am getting very large heats of dilution for the calcium solution in my control tests. We prepared a 1 M CaCl2...

07 February 2021 7,532 1 View

I am looking to develop a basic oral microbiome assay. Can anyone advise on culture vessel, conditions and organisms that require minimal maintenance?

I am looking to investigate oral care products with an easy-to-prepare culture of orally derrived microorganisms.

21 January 2021 3,448 1 View

Cause of blisters in ALD layer at the edge of electrode?

I've encountered a defect during processing that I can't figure out, so I'm hoping I might find some ideas here. My mask design is a microelectrode array, with 60 microelectrodes with diameter...

19 January 2021 665 1 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

What form of data analysis should I use for my pilot study?

I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...

08 January 2021 4,376 4 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Cleaning SGE Micro Channel Device?

Hello all, I have a 3-port SGE microchannel splitter in my Thermo Trace 1310 GC connected to an Isolink 2 combustion interface. My microchannel device is clogged and my flow is significantly...

29 December 2020 7,002 1 View

Weird J-V curve of solar cell?

Recently I am getting low performance (PCE and FF) and weird J-V curves on Pb-Sn-based perovskite solar cells. I have been trying to solve this for a couple of weeks now. I will like any...

19 December 2020 9,336 4 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Should I use nominal or real trade openness index?

Hello every one. At the moment, I am conducting a research (PhD Theses) about trade openness and economic growth in Libya. My question is that, should I use nominal or real trade openness index....

21 January 2021 1,273 6 View

How Islamic State recruited European experts?

I am looking on literature analysing how Islamic State recruited civilian experts as computer technician, experts in managing public goods, doctors, nurses... from Western countries. Is there...

21 January 2021 6,148 10 View

Who knows some kind of legal scheme for renewable energy certificates (REC, iREC, GO) integration requirements?

Who is a "central body" for renewable energy source certification and process implementation? Are REC, iREC, GO securities or there is another legal scheme? Is it possible to trade them outside...

21 January 2021 4,179 3 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Technically is there any difference between policy implications and policy recommendations?

Usually we use the two terms interchangeably in our articles but whether is there any technical difference between them?

26 December 2020 185 18 View

How China is the biggest emitter of CO2 gas in the world?

How China is the biggest emitter of CO2 gas in the world?

25 December 2020 6,184 10 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

Explicit or implicit instruction: The trade-off between feasibility and productivity. Can a language be taught efficiently in an EFL setting?

Teaching realistically in an EFL setting would force us to rely on leaners' explicit knowledge and in turn explicit instruction. On the other hand, long hours of teaching explicitly may not lead...

17 December 2020 9,317 4 View

Will automated killing diminish the error rate by police?

10 December 2020 5,548 3 View