Do you recommend ligation free cloning system?

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Wiwiek Harsonowati

I used topo cloning and the result was perfect! never used abm so can't compare between them. sorry

Anton Slyvka

Hi,

I can certainly recommend SLIC: all you need to care about is proper designing of the primers, and otherwise its very reliable. And you don't need to buy any kits.

https://link.springer.com/protocol/10.1007%2F978-1-61779-564-0_5

Cheers

Mahbobeh zamani babgohari

Wiwiek and Anton thanks for your suggestions.