Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

How can I concentrate peptides?

I am working with an initial volume of 20 mL* and using a 10 kDa filter to separate proteins from peptides. With this filter I am able to concentrate the proteins upstream of the membrane....

15 January 2020 1,520 7 View

Why is one plasmid not transfecting HEK 293t cells , while a simliar second plasmid sucessfully transfects the cells with the same protocol?

I ordered and produced a plasmid from Addgene containing PIK3CA and GFP (pHAGE-PIK3CA-C420R (item #116457). I performed mini- and midi preparations and verified plamid production with a...

02 October 2019 4,078 2 View

Does anybody know the name of this plant from the Canary Islands?

15 April 2019 1,244 6 View

Any data regarding Calcium levels in glioma cells cytoplasm after adenovirus infection?

We are performing experiments on glioma cells. After infection of a cell line (U251), we see that Calcium levels increas even using a mock virus. Any reference that could explain this? Maybe...

03 March 2019 7,772 1 View

Input of WRF wind ouput to a CFD?

Hi. I am trying to a mesoscale and microscale simulation using WRF and a CFD. The output from WRF will be used as the boundary input. I was wondering though since WRF's output for wind is by grid....

21 January 2019 4,990 2 View

How to express units of DPPH radical scavenging activity?

Hi! we are doing some measurements about the DPPH radical scavenging activity of seaweed extracts. We know that the main units used to express this activity are in %. But we have different sample...

31 December 2018 1,744 7 View

How to calculate change in temperature on realistic resistor ?

When using the Specific Heat Capacity ,the following equation provides the temperature change: (T2-T1) = IIRt /(mc) I-Current V-Voltage R-Electrical Resistance t-Time m-Mass of the material c-...

12 December 2018 5,329 2 View

Softened Contact - Exponential - too overclosure. How it is possible?

Hi, It's my first topic so I introduce myself. I'm Iulen, I'm starting a PhD in Zaragoza (Spain). I have created an exponential contact interaction between a deformable body and a rigid body...

09 October 2018 9,097 0 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View