Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

I am proposing to write on effect of stigmatisation and mental illness among BME in Uk. How can I rephrase this pls? Any material on this pls?

I am proposing to write on Stigmatisation and mental illness among the Black and Ethnic Minorities living in the Uk , mostly the Afro-Carribeans. I would like to know how I can re-phrame the...

17 August 2020 3,809 8 View

Species identification from images: Flying fish, cephapods and jellyfish?

Hello there, We have a number of images taken at the surface and during blackwater dives during an expedition to the Sargasso Sea, August 2019. I am seeking experts that might be able to identify...

06 July 2020 4,958 4 View

My logcombiner doesn't work, either version within BEAST 2.5 or 2.6! Any ideas for an online platform, or perhaps a package in R?

I am trying to combine two runs from BEAST 2.6 and strangely my Logcombiner doesn't open. Surely there must be another package that can perform the same function? I have looked for earlier...

19 March 2020 7,701 3 View

What is the rationale of heart rate and temperature monitoring with respect to internet of things?

The relationship that exist between the heart rate ,temperature and internet of things

07 November 2019 7,690 3 View

What caused a western blot to show total protein even after stripping and blocking?

I just finished up a western blot with two specific antibodies (one of which was for tubulin) where, when reading on a 700/800 fluorescent Odyssey scanner, my results look the same as my Coomassie...

03 May 2019 6,286 3 View

How can I further reduce formation of dimerisation/oligomerisation of GPCRs during my Western Blot?

Hello all, I am attempting to compare degradation of a GPCR, specifically the Dopamine D2 receptor, with a mutant form of the receptor I have created, with extended agonist treatment. Both...

24 March 2019 1,058 3 View

How do I respond to full-text requests without posting publicly for everyone?

I received a full-text request through RG and clicked the link from the RG email and it brought me to a prompt where I was forced to either agree to publicly post the paper for everyone to see, or...

21 March 2019 3,017 3 View

How do I unscubscribe?

This is not what I thought Research Gate was. I want to unsubscribe since I have no time for social media. Cheryl

14 February 2019 9,401 1 View

What is a good neuronal apoptotic precursor?

I am trying to find a way to look at damage done after hypoxic stress. I have looked into HIF 1 alpha and the Caspases but I feel like there is a better ones out there that has an early onset and...

13 December 2018 9,872 2 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View

Vth increase with programming SONOS Flash in Tcad?

Hello, I've been working on this issue for more than a month now.... I made a basic SONOS flash structure using TCAD Sentaurus sProcess. (tunnel oxide/nitride/blocking oxide : 4/6/6 [nm]) Then,...

11 February 2021 3,337 3 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View