Usually enzymes are soluble in water, but I am interested some enzymes that function in fatty acids?
I have worked on CANDIDA antartctica Lipase B. Being immobilized on to polymer, it is active in organic solvents as well. It depends on which type of enzyme you are looking for. Some enzymes are active at the aqueous/organic interface to some extent. I have tried alcohol dehydrogenase using aqueous/organic solvent mixture. I got good results depending on the solvent combination and their ratios.
Good luck
Hi, I think majority of lipases in insects are active in fatty acids e.g. Lipase Bromer and TAG-Lipase.
Cheers, A.
No, if you mean a solvent in which no water is present. Lipases need water to work and they will be active at the interface. Almost all membrane bound enzymes or proteins will dissolve in emulsions and will be active at the interface. But if you are thinking of dissolving an enzyme in a pure organic solvent (like benzene or carbon tetrachloride) and expect it to work, then you will be disappointed.
Your best bet is to use a mixed solvent system, preferably in an emulsion. Use a non-denaturing detergent to make an emulsion and the enzyme will work happily at the interface.
But finally it depends on the enzyme and I hope most lipases will work in an emulsion.
http://www.jbc.org/content/218/2/971.full.pdf
Just go to this link
Refer the following links
http://www.jbc.org/content/263/7/3194.full.pdf
Article Improving Enzymes by Using Them in Organic Solvents
Article Enzyme Catalysis in Non-Aqueous Solvents
Fatty acid amide hydrolase, an enzyme with many bioactive substrates. Possible therapeutic implications. . During the last eight years a number of bioactive lipid mediators, the amides or esters of long chain fatty acids, have been discovered or re-discovered. A number of bioactive lipid mediators, the amides or esters of long chain fatty acids, have been discovered or re-discovered. These are: anandamide (N-arachidonoyl-ethanolamine, AEA) and 2-arachidonoylglycerol (2-AG), two endogenous agonists of cannabinoid receptors; oleamide (cis-9-octadecenoamide), a putative endogenous sleep-inducing factor; N-palmitoylethanol amine (PEA), a compound with promising anti-inflammatory and immune-modulatory activity.
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