Do you have any idea on how to standardize the test of Chrome 51 for the cytotoxic activity of cytotoxic T lymphocytes specific for EBV?

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Mariangela Di Bella

Our target is human lymphoblastoid cell lines.

Sarah Matko

Hey Mariangela,

I hope this Website might help you:

http://www.uspbpep.com/usp31/v31261/usp31nf26s1_c1225.asp

For GMP-compliance, you would need to meet all criteria. I would recommend you to perform a spiking experiment. Besides specificity, this might give you an idea about your upper and lower limit of detection.

For further information, just search for the USP (U.S. Pharmacopeial Convention) 1225 paragraph online.

Much of success!