Yes you can use chloroform step in RNAzol

Hello Gabriela,

Yes, Henry Kolge is correct.

You can combine RNAzol with chloroform. You may want to know that there is an improved version of RNAzol called RNAzol RT which uses a unique formulation of phenol and guanidine thiocyanate that effectively separates RNA from other cellular components without the need for a chloroform-induced phase separation to achieve pure RNA extraction.

The chemical composition of RNAzol RT allows for direct RNA precipitation without the need for a separate organic phase created by chloroform in a single step, meaning the process is quick and efficient. 

If you may want to use RNAzol RT, then you may isolate RNA from samples without the use of chloroform in a single step.

Best,

Dear Dr. Kolge and Dr. Noble,

thank you very much for your quick responses.

I usually used RNAzol RT for bacterial RNA exctraction, but there were always some portion of DNA. That´s why I would like to try to add chloroform step. The manufacturer recommands bromanisole. But is it a mistake to choose the addition of chloroform step to better separate RNA from DNA, even if manufacturer declare that it is not necessary, please?

yes, you can use a chloroform step.