We are working on a method for rapid sparse matrix sampling of protein crystals in my lab. This seems to work well with model proteins but we would love to extend it to real-life proteins to test its general applicability. We are looking for 10mg of pure (by SDS PAGE) protein to be shipped to our lab which we would attempt to crystallise. We would like a suitable ligand (e.g. substrate/cofactor) to be included as well as information on the protein. Protein could be fusions and should ideally be cytosolic. We want to assemble a panel of 20 proteins in the first instance.
yes I want to crystallize a protein What do mean by cytosolic how to detect it for a protein
Hi Uday, Thanks for your answer. It is better if the protein is not membrane-associated (either integral or peripherally-associated with a membrane). Such proteins would include receptors, channels or pore proteins. It is notoriously difficult to crystallise such proteins.
Sir My protein is a membrane protein its wolbachia surface protein can we go about for crystallization studies The function of wsp is unknown, and its three-dimensional molecular structure, as well as its precise location in the outer membrane, are yet to be characterized.
Dear David,
I might have some globular proteins for you. Can we talk about it over the email/skype ? so that I know how your approach is different from hamilton standard screens.
P.
Dear Prof. Sheehan,
Good Day!
I have an extracelluar protein (bacteriocin) of about 10.7 kDa, will that work for you?
Hi Afsheen
Yes these would be perfect! Could you send them to me - probably best as lyophilised protein? Dave
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