Hello Yaliang,

for animal tissues, cell cultures and purified mitochondria you could use a commercially available kit, for example:

http://www.biovision.com/succinate-dehydrogenase-activity-colorimetric-assay-kit-7958.html

An alternative kit is available here:

http://www.abcam.com/complex-ii-enzyme-activity-microplate-assay-kit-ab109908.html

Furthermore, there is a recent publication on how to measure SDH activity:

http://www.ncbi.nlm.nih.gov/pubmed/23886887

I hope this information helps you.

Good luck and best regards.

Hi Christian,

Do you know if I can use those methods in plant tissue?

Thank you.

Regards