What are the pretrained-language-model that is obviously better than BERT and RoBERTa?

19 January 2021 602 2 View

Can I design an aptamer that can sense microbial activity?

I am trying to design a boron nitride based biosensor that can sense the abnormality of microbial activities on fish skin. I am very curious that is it possible to have such an aptamer that can...

23 September 2020 6,352 4 View

Can I build a stable cell line for CO-IP?

I want to know two protein interactions via CO-IP, and one of the proteins has several different mutations. I need to transfect all these mutations into HEK293T cells for doing Co-IP every time,...

03 September 2020 342 3 View

Based on transformer, how to improve the text generation results?

If I do not pretrain the text generation model like BART, how to improve the result based on transformer like tensor2tensor? What are the improvement ideas for transformer in text generation task?

18 August 2020 7,265 3 View

Are there any deep models that are better than BERT-CRF in NER task?

Named entity recognition (NER) is task that mark tags of the input text sequence. BERT-CRF is a good NER model. I want to find a better NER model. Or I want to improve the BERT-CRF model. What...

18 August 2020 7,070 2 View

What are the effects of gender of performer on animal?

I remember a paper studied the effects of gender of handler on animal (mouse?) showing that male handler has more negative effects on animal (for example, causing animal anxious) compared to...

05 August 2020 4,131 1 View

Question of pretraining text-generation task, it seems that pretraining is not work for a small model?

My task is to generate keywords from sentences. I pretrain a text-generation model. I mask the sentences' tokens and predict the whole sentences' tokens. Pretraining batch_size = 8 and step =...

28 July 2020 5,675 1 View

How to tackle endogenous problems with "truncated" data structure?

When I want to estimate educational return, I run a regression like: ln(wage)=beta1+beta2*education+other control variables+u In order to tackle the endogenous problems induced by potential...

21 June 2020 2,244 1 View

What is the principle of Unsupervised Data Augmentation (UDA)? Why does UDA work?

UDA(https://github.com/google-research/uda) could achieve good accuracy by only 20 training data on text classification. But I find it is hard to reproduce the result on my own dataset. So I...

05 June 2020 1,486 2 View

What are the simplest methods for the label noise problem?

If I have enough low quality data from unsupervised methods or rule-based methods. Do you think removing the wrong data predicted by trained model is a simple but effective method?

02 June 2020 4,078 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

How long can you store Matrigel-coated Transwell inserts?

Hi, everyone! I was planning to do an invasion assay today and thus coated my inserts yesterday, but unfortunately, due to certain circumstances, I cannot proceed. The next time that I can do the...

09 February 2021 8,771 3 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Spontaneous metastasis of nsg mice reason for early metastasis?

Hello. I have injected breast cancer cells with matrigel in 4th inguinal mammary fat pad of nsg mice. i was not expecting the cells to metastasize before 2 weeks but when I imaged them on day...

31 January 2021 2,817 2 View

What is the effect of room temperature on brain tissue intended for single nuclei RNA sequencing?

I am planning to do single-nuclei sequencing for brain organoids. However, the organoids experienced 25 minutes in room temperature prior to freezing. Most of the time they were in their culture...

17 January 2021 5,257 1 View

How to do immunostaining with 3D culture (tissue embedded in matrigel)?

I dissected tissue from mice then embedded it in matrigel for culture. For analysis, I plan to do immunostaining with some cell markers. But the regular IF protocol did not work. Matrigel would...

11 January 2021 6,961 3 View

Why are the organoid cells showing blue fluorescence even without dapi stain?

Mouse intestinal organoids ; After fixation and storage in PBS-T, when I checked in microscope, the organoid show fluorescent color blue even if I have not added DAPI. Does anyone have such...

05 January 2021 2,067 3 View

Des anybody have a straight forward protocol for invasion assay using colon cancer stem cells? Is there necessary to dilute the Matrigel?

I am working on colon cancer stem cells to see the impact of combinational therapy.

23 December 2020 9,830 3 View

Why the oral cancer cell line from metastatic tongue origin is showing less aggressive property?

I am working with oral cancer cell line SCC090 (ATCC). As per ATCC data sheet it is originated from metastatic tongue. It's primary site is not mentioned. Due to the origination from metastatic...

08 December 2020 5,674 3 View