Do we really need to remove matrigel for organoid (confocal) imaging?

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Malcolm Nobre

Hello Yiling Guo

Not necessary. You may want to refer to the chapter attached below.

Please go through the entire chapter for the protocol.

https://www.sciencedirect.com/science/article/abs/pii/S0091679X22000553

The author has provided a method for immunofluorescence staining and confocal microscopy imaging of colorectal cancer (CRC) patient-derived organoids in basement matrix.

Best.

Chuan Niu

Not necessary. In most situation, the fluorescence signal could pass through matrigel.