Problem with cell growth?

Dear all, I have a very strange problem with my cells (HMLE cells with empty plasmid pBP). They are growing well in the flask, but when I seed them on a 6 or 96-well plate, they don't look very...

23 November 2020 9,904 5 View

ROS detection with DCFH-DA and MitoSOX?

Hi, I am measuring the effect of some compounds on cellular ROS level in HCT116 cell line, and I am wondering is it possible that I detect the ROS increase by MitoSOX, when treating the cells...

15 May 2018 4,934 4 View

How does LPS-dependant activation of monocyte derived DCs work if they don't express CD14 on surface?

I am looking over protocols for differentiation and activation of mo-DCs (analysing with flow citometry). They always use CD14 as exclusion marker (monocytes are CD14+, iDC and mDC are CD14-) for...

26 January 2017 3,383 5 View

Stability of glufosate ammonium on high temperatures?

I was trying to find some data about stability of the phosphinothricin on high temperatures, but I couldn't find much. I am talking about phosphinothricin used for preparation of plates for plant...

17 March 2016 9,481 1 View

Which samplers of soil water are the best for examining the N-compounds in soil water ?

05 February 2015 6,677 10 View

Which samplers are the best for pore water?

Does anybody has experience with sampling soil water with Decagon pore water samplers? Which samplers are the best, if I have to analyze TN, TOC, NO3, NO2, NH4, SO4 from soil water?

22 January 2015 8,835 3 View

Can you help me with a membrane protein (ecto-5'-nucleotidases) solubilisation/homogenisation problem?

I am having trouble with some final steps of my extraction. First of all I need to use a non-ionic detergent for isolation in order to maintain the protein conformation for activity assay. I won't...

11 September 2014 4,177 6 View

Discriminant analysis using SPSS and PAST

Dear all, I performed discriminant analysis using SPSS and PAST software, and I gained the identical eigenvalues for the data set I work with. However, correlations between discriminating...

01 January 1970 4,692 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View