I know that we can observed the production of collagen II and aggrecan for HCS-2/8. I was wondering if it was possible with histological staining (Safranin-O) to see cartilage production?
Abnormalities in the shape and morphology of the red blood cells will provide information in differential diagnosis. According to J. Ford (2013), interpretation of RBC morphologies is very...
06 September 2023 8,394 1 View
Hello everyone, I am trying to isolate bovine monocytes-derived macrophages from whole blood but I have several issues with the protocol: 1) After doing the centrifugation with Histopaque 1.077, I...
30 May 2022 5,743 1 View
Hi everyone We are looking to perform force measurements on hydrogels using an AFM setup. To avoid evaporation, we would like to immerse the hydrogels in liquid, however, the hydrogels may swell...
26 October 2021 6,345 9 View
I found a branch of linden in a grave from the end of the first Iron age. It could have a ritual or symbolic signification. Does someone knows bibliography or comparison about this? Thank you
06 August 2018 4,638 15 View
My aim is to study the interaction of the tidal turbines on the environment, with a focus on sediment in the ocean. Thanks for your advice...
24 June 2018 8,245 6 View
Everybody talks about UAV (personal or Taxi service) being a game changer to our way of traveling and commuting. Looking at ongoing research activities and some early flight demonstrators I...
25 January 2018 6,955 4 View
Knee clearance? Reachability?
20 February 2017 2,961 0 View
I am trying to determine the energetic power of sugarcane straw in my region, but I can't find the biogas potential.
22 May 2014 1,567 3 View
Most of the countries of Africa are affected by water war due to climate change, so let think together how to bring solution for this conflict of interest.
01 January 1970 5,788 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View