Do eukaryotic organisms have reverse transcriptase enzyme?

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Which class of compounds is the best to achieve senescence in cancer cells?

Senescence, defined as the arrest of growth of mammalian cells and prevents unlimited proliferation is of considerable interest in cancer treatment.

02 March 2014 5,210 7 View

Which is a better assay among Alamar blue and MTT?

These assays are used to test cytotoxicity and cytostatic activity

08 September 2013 700 14 View

Can someone name the important pro apoptotic response and the various assays for their quantification?

Time staggered treatment of EGFR inhibitors and genotoxic drugs result in enhanced cancer cell death. Synergistic relation between the drug can be studied by varying the time gap between their...

08 September 2013 9,625 4 View

Can anyone provide the specifications of nutrient medium for the growth of Nostoc , Phytoplankton and Macroalga?

Growth of these compounds can help us to collect useful enzymes by exposing them to UV radiation.

09 October 2012 7,113 6 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

How to estimation of reverse-transcription on tissue?

I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...

08 February 2021 1,957 4 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View