How do I calculate distance between (or precisely the mobility) of a single residue at different timeframes in the trajectory?

Hello, I hope everyone is doing great. I want to calculate distances traveled (from its initial position) by a single amino acid residue at various time frames in the trajectory. To be precise...

05 June 2018 4,100 9 View

How to prepare folic acid decorated nanoparticles for Transmission electron microscopy?

Can anyone please suggest me a way to visualize folic acid (in folic acid decorated nanoparticles) under Transmission Electron Microscopy? Any tagging methods or literature in this regards will be...

09 October 2017 978 6 View

How is a suitable way to prepare folic acid decorated nanoparticles for Transmission electron microscopy?

Can anyone please suggest me a way to visualize folic acid (in folic acid decorated nanoparticles) under Transmission Electron Microscopy? Any tagging methods or literature in this regards will be...

09 October 2017 7,235 4 View

Help please with MATLAB ga toolbox (Not enough input arguments)

Could someone please help me with this, I am trying to minimize a 3 variable problem, and, keep getting this error in return, "Not enough input arguments" Number of variables = 3 Upper bounds =...

06 July 2017 3,396 9 View

Ab initio modelling of polysaccharides from SAXS profiles ?

03 April 2017 7,751 4 View

How is the Amphiphilicity of a polymer estimated?

How can the amphiphilicity of a polymer estimated? Is there any specific methodology to do so? Any mention of particular literature will be helpful and highly appreciated.

05 June 2016 5,689 2 View

Why is Olive oil specifically used for estimation of Oil Holding Capacity?

This experiment is carried out typically for fruit powder. According to a number of researchers, Olive oil is suggested for this experiment. Kindly clarify why only olive oil is preferred?

04 May 2016 4,376 4 View

What do the colors (blue, yellow, etc.) represent in birefringent images ?

01 February 2016 1,665 5 View

How do I rank ligands based on docking scores against different proteins?

Suppose I dock 10 ligands to 5 different proteins. My goal is to say "find out the ligand with the best combination effect". I need my ligand to have maximum docking score against say Protein1 and...

08 September 2015 5,220 6 View

Can you publish a paper using a trial version of some software?

I did some molecular docking work using a 15 day trial version. Now I am confused on whether that work was any worth? Can I publish the data citing the trail software? Please advise.

01 February 2015 3,680 12 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View