Does antibiotic affect the growth rate of resistant bacteria too?

If a bacteria bearing the resistance gene for an antibiotic is grown in presence of the antibiotic, how slow will it be compared to its native form (where it does not have the resistance gene...

06 July 2017 683 8 View

Why is RPMI media's colour is changing so frequently?

I'm using RPMI 1640 media from Himedia. Within 24 hours the colour of the media is changing to yellow even with very low density of cells. I have checked for any kind of contamination, but that is...

04 May 2017 1,335 14 View

What should be the ideal time for batch binding of protein to affinity tagged resin?

I have to purify a bacterial protein from E. coli. My protein's yield is quite low. I have read that batch binding gives better yield, if so, for how much time do I have to incubate my protein...

01 February 2017 3,912 6 View

Does restriction digestion also require supercoiled form of plasmid?

Isolated plasmid when mostly in supercoiled form eases most of the downstream processes, but is it essential for restriction digestion also? If there is less supercoiled and more of nicked...

11 December 2016 8,508 6 View

How stable is the antibiotic ampicillin?

I know that beta lactamase produced by the colonies will degrade the ampicillin in vicinity where spurious satellite colonies can grow but how long can unused plates of Luria Bertani Agar media...

10 November 2016 7,828 9 View

Why do genes cloned in same vector, under same promoter have different levels of expression?

I have cloned my gene of interest (WT) in a vector and in the same vector with same restriction sites I have cloned the point mutated gene. I am not getting any expression for the WT whereas the...

09 October 2016 3,977 7 View

To eliminate the nicked circular plasmids from plasmid prep can ligase be used?

In a plasmid maxi prep, many a times, nicked circular species are isolated apart from supercoiled. Can this nick be rejoined by incubating the prep with ligase? I know it won't increase...

08 September 2016 5,444 6 View

Is there any tool available to determine the protein structure?

07 August 2016 4,768 6 View

How stable are N-terminal truncated proteins?

a. As protein folding generally starts from N terminus. If hypothetically I delete some parts of its N-terminus, will it still be stable and able to fold properly? b. If I delete 30 amino acids...

06 July 2016 5,877 8 View

What is the evolutionary basis of DNA Polymerase being able to synthesise only in one direction?

I feel it contradictory to evolutionary advantage that the two strands of DNA as evolved are anti-parallel but the polymerases that synthesize/ replicate them can do so in only one direction....

06 July 2016 3,598 16 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

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22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

A film coverage that allows for transmittance of wavelengths on the infrared scale?

I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...

08 February 2021 5,760 1 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

How to create a Hypothesis and Thesis in a statement?

03 February 2021 4,496 3 View