How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Has any research on translation investigated the phenomenon of bilingual authors who produce versions of their books in both their languages?

Can the second-language version, strictly speaking, be considered a translation of the first-language version or is it merely a somewhat looser interpretation? How do such authors approach the...

10 December 2020 5,092 25 View

How to distinguish CA1 pyramidal cell vs. interneuron using voltage-clamp?

I am doing voltage-clamp experiments in CA1 pyramidal cells (mEPSCs; AMPA-NMDA ratio; LTP; etc) from acute hippocampal slices in mice. I want to make sure I am only recording from excitatory...

29 October 2020 1,098 2 View

Increase Oleophilicity while decreasing hydrophilicity on silicon surfaces?

Hello RG community, currently I am trying to reach these goals on silicon surfaces for a microfludic project. I have used silanes like APTES in the past in order to get my si-surface hydrophobic...

22 March 2020 2,925 3 View

How could one improve the magnitude of t-statistics of a construct?

Hello All, I am not even sure if this is possible, but I have used this dataset for several publication -albeit with different constructs-and have not seen such low t-values. For example, I have a...

10 March 2020 1,164 3 View

How do I calculate the Mean and Standard Deviation for a set of items (i.e., that form a construct) in SPSS?

Hello All, I have been asked to supply the Mean and Standard Deviation for my latent constructs. Is there a straightforward way of doing it in SPSS? I am actually modeling in SmartPLS, but not...

04 March 2020 1,597 4 View

Any strategies for quantification and purification of a red pigment with no peaks in the visible region which is only soluble in water?

We have isolated a red pigment producing bacteria. We harvested the crude pigment by removing the cells by centrifugation. We tried to find the absorption maxima (lambda max) of the pigment using...

19 January 2020 1,331 2 View

How to Create Small Graphs for Interaction Effects?

Hello All, I have been able to calculate all the interaction effects for a study involving the Big Five. One of the interaction effects I would like to graph (among others) is the relationship...

30 December 2019 6,640 3 View

Can you have stop codon right before a RBS (Shine-Dalgaro) site?

I am designing a primer for a gBlock and I don't want to the poly-histag. So naturally I am adding a stop codon in my primer, but then I need a new RBS site. So my question is can I have a stop...

28 July 2019 1,675 2 View

XML haar cascade for red blood cells and/or echinocytes ?

Hello community, we currently working on an automatic algorithm for erytrocyte detection in stereo-microscopy images for cell counting. Additionally we want to determine the percentile amount of...

23 October 2018 3,262 2 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

ANCOVA controlling baseline in RCT - how to perform power analysis and sample size estimation?

Hi, I have a simple question. I am hoping to perform a power analysis/sample size estimation for an RCT. We will be controlling for baseline symptoms, and using post-treatment or change scores as...

19 February 2021 2,648 23 View

What will happen when we assume an upper bound of OFV lower than the global minimum OFV ?

if we assume 20 is the upper bound of an objective function (OF) based on permutation a square matrix (nxn) , The minimum value of the OF=22, then we run a random operator to compute the OF by...

14 February 2021 6,056 5 View

Pseudo random number generator in Matlab code ?

i want to implement pseudo random number generator in matlab ...i request to every one if someone have code these PRNG share with me SplitMix Permuted Congruential Generator (PCG) Random...

14 February 2021 6,828 7 View

What is the nature of this algorithm?

What is the kind of this algorithm; Input : square matrix Objective function = minimization of total movements cost of matrix based on permutative manner Constrains : Lower and upper bound of the...

04 February 2021 2,050 3 View

How to remove inter-year variation from my assessment of seasonal variation?

I have a 30 year dataset of fish sampling. I know from previous analyses that there is a huge amount of variation between years in catch rates. What I would like to do is create a plot that shows...

01 February 2021 3,980 5 View

Missing not at random imputation?

Hi, I have a case where data is missing not at random... in some cases entire chunks are missing at a time. However, after doing some digging I have found the proper values of some of these cases?...

01 February 2021 945 1 View

How Can we Avoid Overfitting easily in machine learning?

29 January 2021 9,307 6 View