Why is the GFP expressed from my viral vector so weak?

I made a plant viral vector expressing GFP. I got this GFP from a previously made construct (a viral vector of the same type) which I PCR amplified the GFP from, and used restriction enzyme...

19 October 2020 2,863 3 View

Is Intechopen a reputable Publisher?

I have received numerous invites from IntechOpen to contribute to their books. Can anyone assist is this publisher reputable?

08 June 2020 4,217 3 View

How to perfom Unconflated Multilevel analysis?

I want to use Unconflated Multilevel Modeling (UMM) to test a 1-1-1 model in which the effects of the mediating and predictive variables can also be on level 2. Because of a small sample size (164...

24 December 2019 8,603 3 View

Nicotiana benthamiana growth chamber conditions?

UPDATE: SEE MY LAST COMMENT FOR SOLUTION! I'm trying to figure out the best conditions to grow my N. benthamiana plants in a growth chamber. I will be Agro infiltrating them later, around 4...

11 November 2019 6,018 10 View

How to use biotinylated primary antibody; streptavidin APC secondary antibody in compensation beads to get positive population?

Hello, I am currently setting up my compensation matrix in flow cytometry and to do this I am using compensation beads (versacomp antibody capture beads) to get negative and positive population...

13 February 2019 2,224 4 View

How to measure complement receptor 1 per erythrocyte using flow cytometry?

Hello, I am currently looking for a protocol on how to quantitate complement receptor 1 molecules PER red blood cell using flow cytometry. I am aware that in order to do this, a standard curve has...

05 January 2019 5,345 3 View

Plant histology stains?

Hello, I'm looking for plant histology methods for use on traditional microscopes (we have a UV lamp) instead of confocal laser (too expensive for our lab to use) that would allow me to...

18 November 2018 7,647 1 View

How to interpret the following mediation outcome (regression based)?

In my research I hypothised that team learning mediates between transformational leadership and innovative work behavior. To analyse the relations I use HLM 7. I found a significant positive...

25 January 2018 9,913 4 View

Has anyone succesfully expressed Gq DREADDS in rats?

My lab is interested in using DREADDS (Gq) targeting excitatory neurons in rats, specifically Long Evans. Unfortunately, we have not been able to successfully express Gq in cell culture (mice) or...

22 June 2016 8,233 1 View

How long can formalin-methanol fixed cells be in good condition for sorting and further RNA analysis?

18 February 2016 2,358 2 View

What is the role of work experience in motivating high tech entrepreneurship in Aviation industry?

Related work experience plays a vital role in motivating the Entrepreneurship. But when we talk about high tech entrepreneurship in Aviation industry, we talk about very high investment and high...

22 February 2021 376 5 View

Hello, how can I invite an article?

Entrepreneurship in agriculture: a literature review by Roland Condor International Journal of Entrepreneurship and Small Business (IJESB), Vol. 40, No. 4, 2020

23 November 2020 5,898 13 View

Any thoughts for a research topic in a master's level thesis regarding combination of Supply chain and entrepreneurship?

Hi, we are two master's students at JIBS and currently looking for inspiration and suggestions for a research topic that has a connection of both "supply chain" and "entrepreneurship". maybe...

18 November 2020 2,524 3 View

Is the future of "Science" or "the Scientific Implications of Climate Change" connected with the US election?

Donald Trump and his challenger Joe Biden have presented vastly different visions for handling crucial issues, from the coronavirus pandemic to climate change. This results in how the election...

29 October 2020 8,810 6 View

What some of the low-cost with high impact journals one can publish in for Business, Management & Entrepreneurship?

Colleagues, I am looking for advice regarding some of the low-cost but high impact journals for Business, Management, Leadership, Organizations & Entrepreneurship? These could be open...

19 October 2020 2,978 12 View

How is ambidexterity measured on an individual level?

Hello, referring to the concept of Ambidexterity within the business-world/ organizations (e. g. Gibson and Birkinshaw 2004, March 1991, O'Reilly and Tushmann 2013): If one understands...

12 October 2020 5,530 3 View

What are the examples of good professional journals in the areas of management and entrepreneurship?

I am looking for advise regarding good professional (not academic) journals that would publish articles in the areas of management and/or entrepreneurship.

23 September 2020 7,875 6 View

What is the best book to teach entrepreneurship to primary students?

What is the best book to teach entrepreneurship to primary students?

07 September 2020 6,757 3 View

To measure social innovation intent among students what is the right questionnaire and research methodology to publish a quality research work?

I am planning to do research on social entrepreneurship and social innovation intent among youth, especially technical students. In this context is there any standard model that can be tested...

07 September 2020 8,237 4 View

What is the importance of need for achievement as a character of entrepreneurs in understanding entrepreneurship?

The three personality characteristics that are documented in almost all entrepreneurship documented information are: i. Need for Achievement ii. Internal Locus of Control iii. Risk-taking Why in...

17 May 2020 9,849 10 View