What are the recommended kits for cDNA synthesis? Did anyone tried SensiFast cDNA synthesis kit, Bioline? If so, how was it?
You can definitely try ThermoFisher Scientific RevertAid First Strand cDNA Synthesis Kit and also use SuperScript® III Reverse Transcriptase for reverse transcription.
https://www.thermofisher.com/order/catalog/product/K1621
https://www.thermofisher.com/us/en/home/life-science/pcr/reverse-transcription/reverse-transcriptase-enzymes/superscript-iii-reverse-transcriptase.html
Super Script III kit from Thermo Fisher Scientific till date is the best kit to synthesis cDNA.
Hi Ola,
If you want highly reproducible cDNA synthesis for qPCR studies, I would definitely recommend the SensiFAST cDNA Synthesis Kit. We have designed it not only with a buffer that is optimized for high quality, but it also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3' and 5' coverage and reverse transcription of all regions.
We have customers using it for all sorts of studies, from cancer studies to plant germination, let me know what you are doing and I can send you more information. Steve
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