How many raters per word per scale is necessery to obtain a reliable mean value?

In psycholinguistic norming studies 15-20 raters per word per scale are somehow rule of the thumb. However, I cannot find the psychometric explanation or justification for this, although. Does...

27 October 2019 8,716 3 View

Please your experiences with new Thermo Fisher basic PCR instrument: miniamp plus thermal cycler?

We have offer to by this instrument, we would like to decide between thermocycler 2720 and new model. If you could compare it?

23 May 2018 3,253 3 View

Please your experiences with new Thermo Fisher basic PCR instrument: miniamp plus thermal cycler?

We have offer to by this instrument, we would like to decide between thermocycler 2720 and new model. If you could compare it?

04 May 2018 4,065 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Is there a way to cut the restriction enzyme only the desired part?

Hello, I am currently making a luc gene. The primer was ordered and the band amplified with pcr was gel extracted. Restriction enzyme was treated to make the insert sticky with the vector......

18 January 2021 4,357 3 View

Questions on data cleaning and imputation in longitudinal studies?

The study involved mathematics students completing a survey with several scales at three time points. 1) What is a reasonable threshold for missing data to remove an observation? Does this...

04 January 2021 5,582 1 View

Is it possible to ligate two restriction ends those have no complementary bases?

In my lab,we are trying to construct a control plasmid. So we have to remove the insert gene which has two restriction ends:one is XhoI and other one is Hind III.So after double digestion we want...

01 January 2021 3,271 4 View

Do you think knowledge should be in equal access to everyone ?

everyone knows the importance of knowledge but knowing this too why there is a restriction in getting access to it what is reason behind this.

23 December 2020 2,934 6 View