How do you choose the detergent concentration for protein purification?

hey i am using 2%OG and 1%DDM what concentration do i need to use with DM? is there a formula to calculate? Why usualy using 1%DDM and 2% OG (what % is higher then the CMC?) thanks

31 December 2018 3,925 8 View

Is using synonyms or antonyms enough in paraphrasing a text? Is doing this considered Paraphrasing?

When coming to write a research paper or a report, most EFL students tend to paraphrase through using synonyms and antonyms. Thinking that it is enough. How can we deal with such a thing?

26 December 2018 5,894 15 View

How to calculate the sample size? Can I cite the same article(s) in more than one research?

I'm planning to do a research based on questionnaire/ survey and population are clinical researchers from the whole country. The count of researchers in the country in unknown (I don't know how...

07 March 2018 2,405 4 View

In survey, not all questions answered by respondents. What should I do ?

for example, total respondents are 100. 50 answered Q6, 45 answered Q7 , 60 answered Q8. What should I do to write the results to be acceptable for the journal reviewers? The survey has...

04 February 2018 1,164 4 View

When dissolving expanded polystyrene (EPS) in acetone what fumes are produced? And is there a way of measuring this?

I am researching and developing alternate methods of recycling EPS and am getting interesting results via dissolution in acetone. However I want to make an evaluation of the potentially hazardous...

15 January 2018 478 2 View

How do calculate decolorizing activity of bacteria by using uv-vis spectrophotometer?

27 June 2017 596 3 View

How can i produce genomic analysis (DNA extract) with very low concentration of DNA from Yeast?

hey all i need to extract DNA from yeast cells. but how can i do it from one yeast or very low number of yeast cells. is there a special protocol for it? Thanks May

17 June 2017 5,842 9 View

Does exist a gene expression programming toolbox for matlab?

20 May 2017 3,852 4 View

For recharging Hitrap Talon 1ml cobalt, can I use Cl2Co*6H2O?

The GE official protocol says I need 50mM Cl2Co, so should I calculate only by the Cl2Co MW? Thank you!

30 April 2017 3,961 2 View

What settings should i be using on Primer3 and Primer BLAST to prevent primers with potential off-target products from showing up in my results?

22 March 2017 6,290 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

How to remove inter-year variation from my assessment of seasonal variation?

I have a 30 year dataset of fish sampling. I know from previous analyses that there is a huge amount of variation between years in catch rates. What I would like to do is create a plot that shows...

01 February 2021 3,980 5 View