I'm working on a method using Deuterium NMR (2HNMR) to observe different quadrupolar splitting patterns of single phase and multiphase surfactant/D20 solution (lamellar, hexagonal liquid crystals). I have done proton and carbon NMR before but am not very familiar with 2HNMR. Can this be done using a normal solution NMR instrument (Bruker AV 400MHz) or does it require a special probe or a more advanced instrument like solid state NMR to observe the quadrupolar splitting of deuterium nuclei? When preparing my sample for 2HNMR analysis, do I need to dissolve it in any solvent beside deuterated water that was already in the solution, i.e. CDCl3, because some of these solutions are very viscous (hexagonal phase, cubic phase). Any advice would be greatly appreciated. Thank you!
You correctly chose the quadrupole splitting method in the NMR spectra of deuterium nuclei for the study of anisotropic liquid-crystalline phases. The splitting of the doublet is twice as large for the lamellar phase as for the hexagonal phase under identical conditions. We also planned a study using this method. I only saved the next record.
2 H NMR spectra were recorded on a 300-MHz CMX300 spectrometer (Chemagnetics, Agilent, Palo Alto, CA) fitted with a 5-mm 2 H static probe (Otsuka Electronics, Osaka, Japan) using a quadrupolar echo sequence. The 90 pulse width was 2 ms, the interpulse delay was 30 ms, and the repetition rate was 0.5 s. The sweep width was 200 kHz and the number of scans was ~100,000.
Hi Trang, You can use the Lock channel for acquiring the 2H nuclei keeping Lock and sweep off (No need to do ATMA because it will tune at X channel). However you can also use the X channel for observing 2H on the BB probe. Use pulse program zg2H for coupled spectrum or zgig2H for proton decoupled spectrum
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