I used to wrap the bit of tissue in foil, dip in liq. N2 then grind in a mortar and pestle. I unfolded the foil carefully to extract the tissue powder (I needed it to extract RNA from the tissue). I used a ceramic mortar and pestle. If you haven't got one, ask Jon Holdich or Barney, as one might have turned up in the course of the recent lab clear-outs. Hope this helps! Lisa