22 February 2018 3 10K Report

Looking for someone working on Decommoditization of Agriculturey through Identity Preservations and Supply Chain Orchestration.

More Akhil Srivastava's questions See All
Defining effective diffusivity in porous zone and non-porous zone?

Hi all, I am working on methanol steam reforming using packed bed for hydrogen generation. I am using FLUENT software for CFD simulation. In reference paper, authors mentioned that effective mass...

10 February 2021 6,104 1 View

Which pump is used to transfer low melting temperature liquid metal from one container to another?

I want to transfer GaInSn alloy from one container to another with the help of an electromagnetic pump. Can anybody suggest me which brand or which company supplies these pumps?

25 January 2021 9,481 2 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

I'm facing some troubling in designing a anteena ....anyone tell me how give CPW feed in cst ?

I want to give a CPW feed anyone suggest how to providea feed in antenna which I'm designed

18 January 2021 3,517 2 View

Please suggest me suitable journal of Emerald/ Elsevier / Springer for review paper.?

I am working on review paper on " Public Transportation" . Please suggest suitable journal which publishes review paper.

30 November 2020 6,687 3 View

Can any one suggest me when can i upload my paper on public forum as per the general copy right agreement ?

I published the paper one year back and received volume and issue number of my paper from publisher. Can i upload on public forum? please suggest

30 November 2020 1,219 4 View

What is your opinion about zero budget natural farming?

Agriculture is known to provide various innovations from time -to -time , but in the process , we sometimes land in no man's land, and often try to take agriculture to backfoot so miserably in...

06 November 2020 1,910 19 View

How to enforce periodic boundary condition in quantum espresso?

I want to enforce periodic boundary condition in quantum espresso. What changes do I have make in input file?

22 October 2020 6,166 2 View

JMP Pro 14 and one way ANOVA?

I have to analyse the data for one way ANOVA using JMP pro 14 software. Anyone dealt with this software or has any kind of experience. Kindly share it. Thanking you in advance.

23 September 2020 541 6 View

Density measurement during the dilatometry analysis of iron Powder compact?

I am trying to model the sintering process of iron Powder compacts( Fe-1.8%Cr + 2% Ni + 0.5%C ). For starting I am performing the dilatometry experiments of the green compact which would give me...

14 September 2020 6,653 4 View

Similar questions and discussions
How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

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