Dear researchers,what is the quick method to measure the quality of collagenase enzyme?

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Hello,Dear researchers, my question is how to calculate the affinity between antibody and antigen?

My goal is to know what is the minimum volume and concentration of monoclonal antibody that is able to react with a specific volume and concentration of pure antigen?

28 November 2023 4,141 4 View

Hello,Is there a buffer that can be a good substitute for borate buffer?

I want to solve the precipitation of gold nanoparticles in borate buffer with pH 8.4, but I don't have access to this buffer. Is there an alternative buffer that works like this buffer so I can...

14 November 2023 4,091 2 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How does the catalase enzyme contribute to the process of photosynthesis in plants?

كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟

09 July 2024 7,512 1 View

What is the role of catalase enzyme in plants?

ماهو دور انزيم الكاتليز في النباتات؟

09 July 2024 5,824 2 View

What is the protocol for enzyme freeze drying?

what is the protocol for enzyme freeze drying?

09 July 2024 6,464 6 View

How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

08 July 2024 2,285 2 View

Please describe clear and elaborate process of separating solids from garbage enzyme/ eco enzyme after 90 days of fermentation.?

How we should screen out the solid material left after 90 days of fermentation in making of garbage enzyme. Is there any standardized process.

17 June 2024 4,840 3 View

Lenin Kumar Bompalli

Motahare Shahri

SDS PAGE is best.

Other than Enzyme activity assay, Enzyme digestibility assay.

Adam B Shapiro

Collagenase assay kits are available commercially.

https://www.thermofisher.com/order/catalog/product/E12055

https://www.abcam.com/en-us/products/assay-kits/collagenase-activity-assay-kit-colorimetric-ab196999