How to extract peaklists from mass spectrometry raw file and do mascot search for my protein in ncbi?

I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.

30 January 2021 3,081 3 View

How to determine the false positive sequencing result?

Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...

17 January 2021 3,263 11 View

How can i save multi medical images after some preprocessing in a file ?

i have multi x ray images after preprocessing on it , i saved them as a vector in file .dat but this file not accepted in next stage (convolution neural network ). how can i save all image in...

27 December 2020 1,369 4 View

What is microbiology and its types,scope?

the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...

13 December 2020 8,287 6 View

How can one calculate Gibbs energy and entropy in ab initio molecules dynamics?

Hello, I've been investigating a bunch of monomers which spontaneously react in water to form a polymer. I would like to know how to calculate the thermodynamics of such a system. On the surface,...

18 November 2020 5,595 3 View

How to make a solution of resazurin powder ???

I want to test chitosan nanoparticles as antimicrobial against pseudomonas How to use resazurin microtitre assay????

08 November 2020 9,533 2 View

Please suggest me EI compendex conferences with good acceptance rate?

I want to publish a paper at the EI-Compendex conference, please suggest a conference with a high acceptance rate. My field of research is IoT security and cryptography analysis.

19 October 2020 5,365 3 View

Why COS 7 Cells change its morphology to round shape (rounded nucleus and peri nucleus cytoplasm) after Transfection?

I have transfected COS 7cells to express Protein-GFP conjugate, transfection rate was very low (20-30 %) , but the cell morphology changed, many of the cells appear round, (nucleus round too)...

15 September 2020 5,324 3 View

How to separate mixture of two organic compounds using size exclusion chromatography?

My organic compounds have a molecular weight in the range of 2500g/mol and other is 1066.22 g/mol. aThe TLC results show three spots. But the main problem with my compounds are acid sensitivity of...

20 August 2020 6,119 3 View

Dear All. Can normal RNA extraction kit be used for RNA extraction from cell-free medium like culture supernatant, VTM, plasma, etc.?

I am asking specifically for RNA extraction for VTM containing COVID-19 SARS-CoV-2 virus particles. Will the extraction kit make difference in total or specific (viral) RNA yield? There are many...

16 August 2020 8,385 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View