You should follow NCBI

I have had some experience on Realtime PCR. Which actually should be same in principle for different organisms as it works on RNA based quantification.

What is it actually that you are aiming at?

Interesting topic.

Are you doing direct detection from samples? If not, it's as simple as ordinary detection using primer based detection, rt master mix, no probe, non template control, and your positive control or standards, etc. You can also quantify using your standards to comparatively determine the quantity of the genes in your samples. This would be done automatically by your thermocycler, but you have to determine and insert the copy number calculated using the avocadro constant for your standards. Qualifications are only reasonable for sample based detection and less irrelevant for isolate based.