Dear all, can anybody suggest me how to seperate phytoconstituents from alcoholic extract of plant material using column chromatography

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Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View

Does AMBER or CHARMM force field parameters have coarsed grained side chain parameters?

I need to coarse grain the side chain with a single atom. However, what force field parameters do I have to use. Does AMBER or CHARMM have any parameters and partial charges for coarse-grained...

31 January 2021 7,091 3 View

Where can I read the force field parameters of amino acids and protein ?

I am a beginner in the field of molecular dynamics and biochemistry. I need the parameters such as van der Waal radii, partial charge of each amino acid. How do I read directly the parameters from...

20 January 2021 3,541 3 View

Can anyone clarify what is the difference between 'R' software statistics and SPSS Statistics?

I recently learned few things about 'R' programming language. It is open source / free platform in many aspects. R helps to descriptive statistics, inferential statistics, multivariate...

17 January 2021 1,089 20 View

Does anyone know what is the minimum variables required for standard deviation? and why large datasets is considered as reliable data?

For Descriptive statistics, we usually find out mean, median and mode as well as standard errors and standard deviation. Almost in research articles, I can see mean with standard deviation only....

10 January 2021 4,913 3 View

For mineral / compound characterization studies like FTIR, SEM, XRD, how many replicates should be taken for data analysis?

If we do some spectroscopy technique for only one sample, is it enough for data analysis or we have to do multiple samples from each treatment for getting authenticate results to publish in...

09 January 2021 5,896 2 View

What is the reference for the partial charges values for peptides shown in avagadro?

I built short peptides in Avagadro, using Build--->insert--->peptide. After constructing the short peptides of 4 amino acids(tetrapeptide). Under the drop-down, View--->Atomic...

27 December 2020 778 3 View

How to compute the Backward kinematics ?

I have been reading this paper. https://cnx.org/resources/9c9fe5128dfef772b498990a94c49df16c4f09ee/transformation_derivations.pdf Here, they have computed for forwarding kinematics to compute...

18 October 2020 8,208 3 View

How to plot the following XYZ coordinate file?

I am trying to plot the following points in VMD. It is plotting as points. However, It is not connecting the points from A to B to C to D to E to F to H by a line. How do I do it? If not in VMD,...

15 September 2020 4,282 3 View

What is the difference between TURN, COIL and BRIDGE in Protein structure?

I have been reading the following (open access) journal, In this paper, they have collected data samples from the loop region and categorically classified into TURN, COIL and BRIDGE, 3-10 helix...

10 September 2020 4,033 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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03 March 2021 5,978 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

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03 March 2021 8,920 3 View

How to increase a model accuracy ?

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03 March 2021 6,994 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

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02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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02 March 2021 4,359 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

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02 March 2021 2,735 2 View

Jack Silver

Are you looking for antibiotics or determining the activity using bio-assays?

If antibiotics, run TLC plates and do bioautograms.

If an assay, I like to do a column screen (if you don't have an instrument, you can use glass columns and step gradients) with chained gradients. I prefer this method for assay-directed fractionation since TLC may resolve visible bands, which may (or may not) be active compounds.

http://www.teledyneisco.com/en-us/liquidChromatography/Chromatography%20Documents/Posters/New%20Techniques%20in%20Extract%20Column%20Screening%20Poster.pdf#search=column%20screen%20poster

http://www.teledyneisco.com/en-uk/liquidChromatography/Chromatography%20Documents/Posters/New%20Techniques%20in%20Extract%20Column%20Screening%20II%20Poster.pdf#search=column%20screen