Dear all, has anyone used ddPCR (digital droplet PCR) for assessing fungal communities? Could you recommend any specific primers? Thank you in advance, Respectfully, Tanya
Hi Tanya, you can find rare species of interest with this technology. You need to have species-specific sequence ( it could be species specific gene i.e. gene responsible for specific metabolite production) or it could be a variable on species level sequence ( may be specific primer and probe for spacer or intron region). And then you can design you primers and probes.