23 February 2017 1 5K Report

Also are there open data sets for the basis of business intelligence in agriculture.

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Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

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RIPA content? any additional suggestion?

i am working on phosphorylation of NF-kB inducible in hypoxia using MDA-MB 231 cells. i have challenges getting my phosphoproteins through western blot. this is my RIPA content 50 mM Tris pH...

13 November 2020 3,356 3 View

Differences between All atoms Molecular Dynamics simulations and Coarse Grain Dynamics Simulations?

Hi everyone, Please, I need someone to tell me more about the strength and limitations of Coarse Grained Molecular Dynamics simulation. I read about the strength and its incredibly fascinating how...

15 September 2020 7,487 2 View

I am proposing to write on effect of stigmatisation and mental illness among BME in Uk. How can I rephrase this pls? Any material on this pls?

I am proposing to write on Stigmatisation and mental illness among the Black and Ethnic Minorities living in the Uk , mostly the Afro-Carribeans. I would like to know how I can re-phrame the...

17 August 2020 3,809 8 View

Species identification from images: Flying fish, cephapods and jellyfish?

Hello there, We have a number of images taken at the surface and during blackwater dives during an expedition to the Sargasso Sea, August 2019. I am seeking experts that might be able to identify...

06 July 2020 4,958 4 View

My logcombiner doesn't work, either version within BEAST 2.5 or 2.6! Any ideas for an online platform, or perhaps a package in R?

I am trying to combine two runs from BEAST 2.6 and strangely my Logcombiner doesn't open. Surely there must be another package that can perform the same function? I have looked for earlier...

19 March 2020 7,701 3 View

What is the rationale of heart rate and temperature monitoring with respect to internet of things?

The relationship that exist between the heart rate ,temperature and internet of things

07 November 2019 7,690 3 View

Can i use same primers of a carboxylesterase gene in Locusta migratoria for that same gene in Zonocerus variegatus. Will the PCR work well?

ways to use the same primers of one organism for closely related one.

12 August 2019 2,085 4 View

What caused a western blot to show total protein even after stripping and blocking?

I just finished up a western blot with two specific antibodies (one of which was for tubulin) where, when reading on a 700/800 fluorescent Odyssey scanner, my results look the same as my Coomassie...

03 May 2019 6,286 3 View

How can I further reduce formation of dimerisation/oligomerisation of GPCRs during my Western Blot?

Hello all, I am attempting to compare degradation of a GPCR, specifically the Dopamine D2 receptor, with a mutant form of the receptor I have created, with extended agonist treatment. Both...

24 March 2019 1,058 3 View

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