How to do MD simulation of docked protein complex after docking .?

I have perform some protein-protein docking studies. I used Hdock, Prizm. But I am unable to find a proper method to verify my docked structure. I also docked my structure in Hex program to get...

19 October 2020 4,083 7 View

How can we measure the content of happiness in a person by applying various mathematical and soft computing techniques?

Here I just want to know about the actual parameters to measure the content of happiness in a person. With the help of these parameters a neural network can be generated and maintained to achieve...

22 September 2020 7,666 2 View

Which tool to use for Sample Size Calculation studies for AI based studies involving Machine learning or Deep Learning?

I want to know of an online tool or formula to give a vague idea about minimum number of enrolees for a study involving use of machine or deep learning.

01 August 2020 747 3 View

What could be the potential reasons for a long DNA molecule not able to attach to the mica surface for AFM imaging even in the presence of Mg++ ?

29 June 2020 2,075 2 View

What is the best anionic surfactant to disperse TiO2 nanorods to achieve stable aq. suspension?

I have tried PAA and F-127 to disperse TiO2 nanowires after aggressive probe-sonication which resulted in smaller aggregates upto few microns instead of individually dispersed as seen from DLS,...

04 May 2020 7,516 3 View

How Machine Learning Algorithms can be applied for the High Altitude Wetland restoration? Any idea how ML can be integrated with Geospatial Tools??

Wetland Extent Mapping

09 February 2020 3,921 3 View

Not Getting Clone/Colony with NEB HiFi Assembly Cloning Kit ?

In short, I am trying to get a clone of 1664 bp insert in a mammalian expression vector (Size of vector is 6250 bp) by using NEBuilder HiFi DNA assembly Cloning kit with commercial Comp cells...

06 January 2020 541 3 View

How to find gene with similar function across Bacteroides and Firmicutes?

My genes interest is EntA in E.coli. I want to find genes with similar function like EntA in any members of Bacteroides and Firmicutes. I need the DNA sequence of EntA-like genes in these phyla.

01 January 2020 2,400 1 View

Biofilm Fixation for Confocal?

I have grown biofilm and I need to fix it and stain it. 1. Clean the entire disk carefully and aseptically with pipette so that extra biofilm which are present over the walls. 2. Cells are fixed...

17 July 2018 3,954 5 View

What would be the best for miRNA NGS validation?

I would like to validate miRNA NGS data by qRT-PCR. This theoretically could be done from total RNA samples (the one used for sequencing purpose).What approach should I use to isolate the miRNA or...

25 February 2018 4,574 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View